SubtiBank SubtiBank
yhdT [2019-08-23 13:26:17]
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yhdT [2019-08-23 13:26:17]

unknown
locus
BSU_09590
pI
4.75
mw
51.35 kDa
protein length
461 aa Sequence Blast
gene length
1386 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.1|Magnesium uptake/ efflux]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,035,554 → 1,036,939

    The protein

    Protein family

  • [SW|UPF0053 family](according to UniProt)

    • Paralogous protein(s)

  • [protein|BE0777DE7C40825D4DB7252EA84AAB3892578529|MpfA], [protein|E53B8D437B848B637AEC8C1BB9428EC8B6EB280E|YqhB], [protein|45F03AB13292BBF0554785C7C02FE29033EDD742|YrkA], [protein|A459410312A926365B45CEB4694DE399B38820A2|YugS]

    • [SW|Domains]

  • [SW|DUF21 domain] (7-202)
  • 2 [SW|CBS domain]s (221-280, 290-347)

    • Structure

  • [PDB|4HG0] (CorC from E. coli, corresponds to the [SW|CBS domain]s and the C-terminal transporter-associated domain)

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|B2E9D026B668C8530C406353E3400059388566FB|yhdT]
    description
    [Pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-A698 (yhdT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/698 NBRP B. subtilis, Japan]
  • BKE09590 (Δ[gene|B2E9D026B668C8530C406353E3400059388566FB|yhdT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTAACCCTATCCTCTCA, downstream forward: _UP4_AGCGAGAAATAAGCTCAAGG
  • BKK09590 (Δ[gene|B2E9D026B668C8530C406353E3400059388566FB|yhdT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTAACCCTATCCTCTCA, downstream forward: _UP4_AGCGAGAAATAAGCTCAAGG

    • Expression vectors

  • pGP2928 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • References

  • 20525796,27120414,27933050